Release of (A) ³H-serotonin, (B) IL-6, and (E) TNF-α (all shown as mean ± SEM, n = 4 to 5 per point) or expression of mRNA for IL-6 (C and D) or TNF-α (F and G) by rrSCF or IgE/DNP-HSA-stimulated BMCMCs derived from WBB6F₁-Kit^W/Kit^W-v mice or the congenic +/+ mice. (A) Release of serotonin (assessed as percentage of specific release of [³H]-5HT) at 10 minutes after challenge. (B) IL-6 production 2 hours after challenge. (C) Northern blot of IL-6 mRNA induction 2 hours after stimulation. (D) Densitometric analysis of IL-6 mRNA expression, presented as the ratio of density of IL-6 mRNA and G3PDH mRNA in the same specimens shown in (C). (E) TNF-α production 2 hours after challenge. (F ) Northern blot of TNF-α mRNA induction 2 hours after stimulation. (G) Densitometric analysis of TNF-α mRNA expression (ratio of density of TNF-α v G3PDH mRNA) in the same specimens shown in (F ). †P < .001 versus values for Kit^W/Kit^W-v BMCMCs.