Discrimination between CD14⁺ cells isolated from controls or Gaucher patients. PBMC were isolated from two controls and two unrelated Gaucher patients, incubated for 30 minutes in the presence of 1 mmol/L PFBFDGlu, and stained with αCD14 MoAb. Preincubation in the presence of the specific inhibitor CBE was conducted for 1 hour, as described. Dead cells were excluded by software gating on the basis of PI staining. Greater than 90% of cells were viable in all samples analyzed. Ten percent probability contour plots of CD14 versus fluorescein are shown for PBMC incubated in the presence or absence of substrate. The MF values of the CD14⁺ populations within each sample are displayed in the upper right corner of each plot.