Kinetics of IL-12 p35 and p40 mRNA accumulation in monocytes. Monocyte-enriched adherent cells were cultured with SAC. Cells were harvested for mRNA assay at various times after the beginning of the culture. mRNA is expressed as normalized femtograms of IL-12 p35 and IL-12 p40 cDNA. For normalization, the IL-12 p35 and p40 mRNA amount in femtograms was divided by femtograms of GAPDH. Each point plotted represents the mean ± standard error of mean (SEM) of quadruplicate culture samples. This experience is representative of three independent experiments.