(A) Sequential analysis of proviral HTLV-I integration in patient 1. Cellular DNA of MNC was digested by EcoRI and hybridized with the pX probe. Two bands larger than 9 kb, (M type), were detected in LN at onset (lane 1), while same sized M type bands, distinct from those in lane 1, were detected both in PB at onset (lane 2) and in PB at relapse (lane 3). (B) Sequential analysis of TCRb gene rearrangement with HindIII in patient 1. (C) Control DNA from HL-60. Samples in lanes 1 to 3 represent those in lanes 1 to 3 in (A). Lane 1, one rearranged band, lanes 2 and 3, two rearranged bands of the same size that were distinct from the rearranged band in lane 1 (GL, germ line; R, rearranged band). (C-1) Sequential analysis of proviral HTLV-I integration in patient 2. One band >9 kb, (C type) was detected in all PB during the course with the pX probe, while one band <9 kb, (D type) was detected in LN at crisis (lane 7). (C-2) Samples in lanes 1 to 8 represent those in lanes 1 to 8 in (C-1). With the gag-pol probe, the same size of C type band as that shown in (C-1) was detected in lane 1 to 6 and 8, while the D type band with the pX probe was not detected with the gag-pol probe in lane 7. (D) Sequential analysis of proviral HTLV-I integration in patient 3. The same C type band was detected in PB at onset and at remission (lanes 1 and 3) with the pX probe. M type bands, of a size distinct from the C type, were detected in LN at crisis (lane 2). (E) Sequential analysis of proviral HTLV-I integration in patient 4. The same size of M type bands was detected at onset (lane 1) and just before chemotherapy (lane 2) in PB with the pX probe. The density of one of the two bands decreased and that of the second increased at crisis (lane 3), whereas the second was only detected at the terminal stage (lane 4). (F) Sequential analysis of TCRb rearrangement with HindIII in patient 4. Samples in lanes 1 to 4 represent those in lanes 1 to 4 in (E). One rearranged band of the same size was detected in lanes 1 to 4.