Fig. 2.
![Fig. 2. Partial thrombin digests of [3H]NEM-derivatized, Ca-TSP. One hundred twenty micrograms/milliliter Ca-TSP was derivatized in 6 mmol/L EDTA at 24°C. The label was removed by dialysis overnight as described in Materials and Methods. The labeled Ca-TSP was incubated in TBS containing 6 mmol/L EDTA with 120 nmol/L thrombin at pH 8.0 for 1 and 3 hours. The fragments were analyzed with a reducing, 5% to 20% gradient gel (A) or with a nonreducing, 5% to 15% gel (B). The gels were stained, destained, and prepared for fluorography. Fluorographs are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/9/10.1182_blood.v89.9.3205/3/bl_0049f2.jpeg?Expires=1735827231&Signature=vfwht8JbK5NFXyLVzW6LZ502WA4CM3qHpgs3uV3cxy1gJezJstjkn-6nJfxnNOUQLlmDUwd~DHSBBfZ0JhMI1XuyICpQgAijt90dN7msQ0h~bX5cVMlGfBh99dqsPJHQlXc8O3GZQIGdjyY8jcA~OA5BqW5~WJXiyLH-Xe-vuer1O1sYksID3oe3W5qiyRqoLnt5Eh-veE24aGRe3IiQakIYA0NI7OVzJItpC4NF6Ag9xQ66YTrpqzBVPPvowdRU5m5YjICyYN7aGp4DYcG64~XkrQXi30409bgiPTE1SoInSt3nTKMWeBlGhTAa6mEGRFWZIqbkolYuaXG0jDYNMQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Partial thrombin digests of [3H]NEM-derivatized, Ca-TSP. One hundred twenty micrograms/milliliter Ca-TSP was derivatized in 6 mmol/L EDTA at 24°C. The label was removed by dialysis overnight as described in Materials and Methods. The labeled Ca-TSP was incubated in TBS containing 6 mmol/L EDTA with 120 nmol/L thrombin at pH 8.0 for 1 and 3 hours. The fragments were analyzed with a reducing, 5% to 20% gradient gel (A) or with a nonreducing, 5% to 15% gel (B). The gels were stained, destained, and prepared for fluorography. Fluorographs are shown.
