Fig. 3.
![Fig. 3. Effect of temperature and pH on derivatization of TSP with [3H]NEM. One hundred fifty micrograms/milliliter Ca-TSP was incubated at 37°C for 30 minutes or adjusted to pH 8.4 with 0.1 mol/L Tris base before derivatization with [3H]NEM. Limited thrombin digestion, SDS-PAGE on 5% to 20% gradient gels, and fluorography were as described in Materials and Methods. Shown here are fluorographs of undigested TSP (0 hours) and 3-hour thrombin digestion in 6 mmol/L EDTA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/9/10.1182_blood.v89.9.3205/3/bl_0049f3.jpeg?Expires=1735505477&Signature=aAFHt4qla-nvqSYpOVJT5sLIhOtAhFMkWOpMkWgkB0gJg27rHUAGnjH7bqk4xTRy5G0GVo1Eb9SW2ytNp7h2LKLqCcdCH8ci~3VRQrm2rVkLHzJf5llP5u51QpB88wnAQ2cm4MvxFIIxByfswTTZJ6Wkk2sQbYttlYdO1NXIZY1MxzrxwKDdxx5FRh0Kcl23YiWcWw76taV99BlDkWq4cBZ8R-yJOr757fcPOxqbLV~YKosCH57CeY1zYSvvMIkGhe8o1EuybWBq0IT92Ctq-3759fUgTT9R2BYeP0w2nFx1b7om~eQR9jzwxG~wuxt2bfP-2g6QTWtE7ZyKDPM~Ww__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of temperature and pH on derivatization of TSP with [3H]NEM. One hundred fifty micrograms/milliliter Ca-TSP was incubated at 37°C for 30 minutes or adjusted to pH 8.4 with 0.1 mol/L Tris base before derivatization with [3H]NEM. Limited thrombin digestion, SDS-PAGE on 5% to 20% gradient gels, and fluorography were as described in Materials and Methods. Shown here are fluorographs of undigested TSP (0 hours) and 3-hour thrombin digestion in 6 mmol/L EDTA.
