Fig. 4.
![Fig. 4. Effect of PDI on derivatization of TSP with [3H]NEM. One hundred microliters of purified platelet PDI (43 μg/mL) was incubated with 200 μmol/L GSH for 20 minutes before the addition of 0.9 mL 150 μg/mL Ca-TSP. After 30 minutes, TSP was derivatized, digested, and analyzed as described for Fig 3. Shown here are the fluorographs of the reduced gels of undigested TSP (0 hours) and a 3-hour thrombin digestion in 6 mmol/L EDTA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/9/10.1182_blood.v89.9.3205/3/bl_0049f4.jpeg?Expires=1735824365&Signature=Bh2HKIGGKabM1oTmLpZdnPntJa04aLlERJs5~IwLqfm9GRWOLe~YjibJXm2q5z2PLJCkm7SK1o2InjDjek10VQSCphi~Va8cdDeX584q2ZiYRi~-Zl6lQkIAA18bJPo1WRrQGxXh34D2tt0MP7FnSaFMrSKCDe3UChv2D5dwVDmaYgvKuA4LovKDYJh7YglSry6NRwYE9ztrgsaHYjOdItEc1fXZJZtUTemZMaWHP0bXlK0M6DGW5eU1EVvYLu0x3kVQITd8iAJ8WyW8yr4N39WHtB7ZXTyszdrwFRWCZuO~KiJIMBJtLJeASQOJckHZtjEPoPEBwjEUyVgnrLFvpg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of PDI on derivatization of TSP with [3H]NEM. One hundred microliters of purified platelet PDI (43 μg/mL) was incubated with 200 μmol/L GSH for 20 minutes before the addition of 0.9 mL 150 μg/mL Ca-TSP. After 30 minutes, TSP was derivatized, digested, and analyzed as described for Fig 3. Shown here are the fluorographs of the reduced gels of undigested TSP (0 hours) and a 3-hour thrombin digestion in 6 mmol/L EDTA.
