Fig. 1.
![Fig. 1. IL-3, Epo, and Tpo activate both JNK1 and JNK2 in in vitro kinase assay. FDC-P2 cells (upper panel), FD-EPO cells (middle panel), and FD-TPO cells (lower panel) were stimulated with IL-3, Epo, and Tpo, respectively, for the indicated time up to 120 minutes. JNK1 (A) and JNK2 (B) activity was measured in the immunoprecipitates with anti-JNK1– and anti-JNK2–specific antibody and [γ-32P]ATP and GST-c-Jun as a substrate. Arrows indicate the phosphorylated GST-c-Jun (molecular weight, 35 kD).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2664/4/bl_0055f1.jpeg?Expires=1735529677&Signature=B6lSuk9htbfKItYZkUGV3Zbo427u4YE827Sw3s2Ey9TlFA3TeWtb48LS6EMnuRO8lXed3uQhJbYyrfDSdegp6T-MxCIBRaqqJcraWLK~aZCIfBfvjXw9hly0D~FYDG~vUICr2YSiACwqXpurE6uQeiQ63ZIevNgXCBKZBYv~rpkQue6VunQ6i~EcuLBsXl-7muPYjcjxuCZhP6wnU4EAyuMGot-~Ke-5~1M9LxqtYaedLqHqDtFFsnY94UAYbyoWKLoPZuFE9dcTHZQYQDGD~ITcGt~4eXzsUtJIv9ruBv4JYhowzo-0AoGoc5E0WJf-94gMrcnvlPpkiK6YqAs3yQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-3, Epo, and Tpo activate both JNK1 and JNK2 in in vitro kinase assay. FDC-P2 cells (upper panel), FD-EPO cells (middle panel), and FD-TPO cells (lower panel) were stimulated with IL-3, Epo, and Tpo, respectively, for the indicated time up to 120 minutes. JNK1 (A) and JNK2 (B) activity was measured in the immunoprecipitates with anti-JNK1– and anti-JNK2–specific antibody and [γ-32P]ATP and GST-c-Jun as a substrate. Arrows indicate the phosphorylated GST-c-Jun (molecular weight, 35 kD).
