Fig. 2.
![Fig. 2. IL-3, Epo, and Tpo specifically activate both JNK1 and JNK2. FDC-P2 cells (left 2 lanes), FD-EPO cells (middle 2 lanes), and FD-TPO cells (right 2 lanes) were stimulated with IL-3, Epo, or Tpo, respectively, for 0 minutes (−) or 15 minutes (+). In-gel JNK assays were performed in the immunoprecipitates with anti-JNK1 (A) or anti-JNK2 (B) antibody. The immunoprecipitates were separated by SDS-polyacrylamide gels containing GST-c-Jun and incubated with [γ-32P]ATP after protein renaturation. The phosphorylated GST-c-Jun corresponding to the molecular weights of JNK1 (46 kD) and JNK2 (55 kD) are indicated by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2664/4/bl_0055f2.jpeg?Expires=1735846549&Signature=eOYhKQhScBTAKtdJps5Ec1vBOU50duJg0In7t20zJKevrwUlQLzKYzPtaaCefsogsf45A5uy-tAICXG4IjbSBRS4UGf4ZcgmVB4-g8lhwT3IF4eC-4BrJrNTMn6R9dGQu-hPl7YEahLRLvryJmh-cnz9AqJRVym7MpAYGpZAiNtblt9VDQiL9st6sXbXASWmC2PrkbIJUal8ShcyQu-Q9-aB-509oQkPV~hvBkEZ~gi805VY6GbSESRN3zZFZq~hM9MGctqvI5e376WldkmPArNN~YpNIM6d9KadveR7yMmCt8coco5DbEmiE8jIdX6Tqbk5GvqW9zqqeX-xXhaYJg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-3, Epo, and Tpo specifically activate both JNK1 and JNK2. FDC-P2 cells (left 2 lanes), FD-EPO cells (middle 2 lanes), and FD-TPO cells (right 2 lanes) were stimulated with IL-3, Epo, or Tpo, respectively, for 0 minutes (−) or 15 minutes (+). In-gel JNK assays were performed in the immunoprecipitates with anti-JNK1 (A) or anti-JNK2 (B) antibody. The immunoprecipitates were separated by SDS-polyacrylamide gels containing GST-c-Jun and incubated with [γ-32P]ATP after protein renaturation. The phosphorylated GST-c-Jun corresponding to the molecular weights of JNK1 (46 kD) and JNK2 (55 kD) are indicated by arrows.
