Fig. 4.
![Fig. 4. IL-3, Epo, and Tpo did not activate SEK1/MKK4. FDC-P2 cells (lanes 1, 2, 7, and 8), FD-EPO cells (lanes 3 and 4), and FD-TPO cells (lanes 5 and 6) were stimulated with (+) or without (−) IL-3 (lanes 1 and 2), Epo (lanes 3 and 4), Tpo (lanes 5 and 6), and sorbitol (lanes 7 and 8), respectively. SEK1/MKK4 activity was measured in the immunoprecipitates with anti-phospho–specific SEK1/MKK4 antibody and [γ-32P]ATP and GST-JNK1. An arrow indicates the phosphorylated GST-JNK1.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2664/4/bl_0055f4.jpeg?Expires=1735530945&Signature=0gNLvkd4TqfLnM2r2GSIHdWs~uI5AWpCePIhcK4KRiNJtmaguMVdthVhaZLF0MUjXnt9sMWTNBSMH-j5eKpvicfBUfYo8QVVR-0KNzZ6BPBDOo5BMTBBEPt9aq8ON9rCNjw2wzvzlcDKFXlf0XBWA8Cgi3d4VDN7IcjCJwBjdld6b0iLgZFuCbuZ1k8Tdl3bHbi7OUdf0r0fWUoZ6Vamc-X2ZDXsN1yx-mISWKIgavVZn85ssHfgEMKL38PtLDLBnOfEAAwV1aM7m~ImhAKgd6wPj3qEtlq4GxoyGAkk5KDPADUJbcAU9vyZk7O3kFFFKvAHWb8Q7TbdDKre2sx1xw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-3, Epo, and Tpo did not activate SEK1/MKK4. FDC-P2 cells (lanes 1, 2, 7, and 8), FD-EPO cells (lanes 3 and 4), and FD-TPO cells (lanes 5 and 6) were stimulated with (+) or without (−) IL-3 (lanes 1 and 2), Epo (lanes 3 and 4), Tpo (lanes 5 and 6), and sorbitol (lanes 7 and 8), respectively. SEK1/MKK4 activity was measured in the immunoprecipitates with anti-phospho–specific SEK1/MKK4 antibody and [γ-32P]ATP and GST-JNK1. An arrow indicates the phosphorylated GST-JNK1.
