Fig. 3.
![Fig. 3. Expression of HV3, RSA, and fusion proteins. (A) Schematic, linear representations of the recombinant proteins produced in this study. Hatched areas represent HV3 segment, while solid areas correspond to RSA segments. L1, linker 1 [(G)6SM]; L2, linker 2 [MV(G)6 ]. Size of proteins, in amino acids (aa), is given at right. (B) An anti-RSA immunoblot showing immunoreactive components of conditioned media from Cos cells transfected with the following plasmids: pSG5-RSA (RSA), pSG5-HLA (HLA), pSG5-ASR (ASR), pSG5-ALH (ALH), and pSG5 lacking any insert (CON). Horizontal marks, at left, indicate the positions of the 94-, 67-, 43-, and 30-kD molecular-weight markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/9/10.1182_blood.v89.9.3243/3/bl_0055f3.jpeg?Expires=1735543773&Signature=weB3CO4AXP1i35TbmX4vKzkc2ytQauITbOCFbghv~Ve4jAlk2sWvEuDwMlK9AhRj025aRpq8zvlJsne1YIKNy0s75Odt6UPzRjHHwU11dfx2tliZd5HjERXtOqPZtamxj5k8Vq0kibAFHG2SVJVWCGlAX5siz9hYzdyklQAdrCzrale6UyklKUf3xtx5yAMECsLOFgT7r1pEyDDogr10k~xDLcDSSZPCD9txHy8bP1Hl-V0SsvHHXjnv83sP1gGwvI2HO6W0yMzSr3h410GLqSFJy-knYXuBcVYCDxdNUur5r5fpAB3P7E17kGZrpG4pTN6Ely6I0DKtJuNp4RXVhg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of HV3, RSA, and fusion proteins. (A) Schematic, linear representations of the recombinant proteins produced in this study. Hatched areas represent HV3 segment, while solid areas correspond to RSA segments. L1, linker 1 [(G)6SM]; L2, linker 2 [MV(G)6 ]. Size of proteins, in amino acids (aa), is given at right. (B) An anti-RSA immunoblot showing immunoreactive components of conditioned media from Cos cells transfected with the following plasmids: pSG5-RSA (RSA), pSG5-HLA (HLA), pSG5-ASR (ASR), pSG5-ALH (ALH), and pSG5 lacking any insert (CON). Horizontal marks, at left, indicate the positions of the 94-, 67-, 43-, and 30-kD molecular-weight markers.
