Binding of selectin antibodies to COS cells transfected with E-selectin constructs. E-selectin MoAbs 9A9 (B), 10E9.6 (C), and 10E6 (D), but not P-selectin MoAb 5H1 (E) or L-selectin MoAb MEL-14 (F ), bind to COS-cells transfected with a cDNA encoding for the lectin (Lec), EGF, and CR1-6 domains of murine (Mu) E-selectin (top). Negative control antibody shows no binding (A). MoAbs 9A9 and 10E6, but not 10E9.6, bind to COS cells transfected with a chimeric selectin containing murine lectin and EGF domains and human (Hu) CR1-2 domains (middle). Identical results to those seen with the murine/human chimera are seen with COS cells transfected with truncated mouse E-selectin (lectin and EGF domains only; bottom).