Attachment, rolling, and detachment of 32Dcl3 cells to adsorbed murine E-selectin fusion protein under flow. Cells were injected at a wall shear stress of 0.8 dyn/cm2 at time 0. The number of adherent and rolling 32Dcl3 cells in the absence of antibody (□) was not changed by 10E9.6 (⋄), but was almost completely abolished by 9A9 (○) (B). Mean ± SD of multiple fields. Rolling of 32Dcl3 cells (A) or HL-60 cells (C) on adsorbed murine E-selectin fusion protein in the presence of laminar flow (0.8 dyn/cm2 ) was expressed as number of cells per square millimeter at 5 minutes after onset of perfusion. Data are presented as the percentage of adherent cells in the absence of antibody. EDTA shows divalent cation dependence of adhesion, 9A9 almost completely blocks adhesion, and 10E9.6 has no effect. Mean ± SEM of multiple fields in two to three independent experiments. (D) Once engaged in rolling adhesion, 32Dcl3 cells are extremely shear resistant. Data are expressed as the percentage of bound cells at 0.8 dyn/cm2 after shear stress increments of 1.6 dyn/cm2 at 30-second intervals. Cells are not detached up to a shear stress of 9.6 dyn/cm2 either in the absence (□) or presence (⋄) of 10E9.6. Mean ± SD of multiple fields.