Expression of E-selectin in cremaster muscle venules of C57BL/6 and Balb/c mice. Mouse cremaster muscle whole mounts stained with immunoperoxidase as described in the Materials and Methods. Negative controls include specimen injected with 10E9.6 against E-selectin, but not treated with TNF-α (A) and TNF-α–treated specimen with irrelevant rat IgG2b (B). Strong E-selectin expression shown in venules of cremaster muscle obtained from C57BL/6 mice treated with TNF-α for 3 hours and injected with E-selectin MoAb 10E9.6 (C). Under the same conditions, similar staining was observed in Balb/c mice (D). Arterioles showed no detectable E-selectin expression under the same conditions. Bar represents 50 μm.