Representative bisulfite sequencing autoradiogram showing cloned inserts of PCR-amplified bisulfite-treated DNA from PBMCs from donor 2. Examples of cloned inserts with and without methylation of CpG sites in the EBNA-2 responsive region of the C promoter are shown. Arrows indicate CpG dinucleotides. ‖─, a Taq polymerase error. Unmethylated CpG sites are displayed as TpG as a result of the bisulfite treatment, whereas methylated CpG sites are displayed as CpG. CpG sites are numbered as in Table 1. Site no. 1 is not shown. The vector/insert boundary is denoted as vector.