Immunochemical characterization of huHCA-Fc and raHCA-Fc fusion proteins. CD14-Fc, raHCA-Fc, and huHCA-Fc fusion proteins were purified as described and coated onto an ELISA plate at 10 μg/mL. Samples containing MoAbs (▪) 11-59, (▨) F84.1, or (▥) relevant controls (10 μg/mL IgG1) were added, followed by alkaline phosphatase conjugated with antimouse Ig for 1 hour. p-nitophenylphosphate was used to develop the ELISA and results were read at 405 nm using an ELISA reader.