Immunoprecipitation of recombinant huHCA-1, huHCA-2, and raHCA transfected COS-7 cells with F84.1 and 11-59. COS-7 cells were transiently transfected with expression vectors encoding each HCA cDNA or with empty vector and immunoprecipitation and gel electrophoresis was performed as described in the Materials and Methods. A 3-day exposure of the dried gel is shown. The positions of molecular weight markers are shown at left.