Fig. 1.
![Fig. 1. Autoradiograph of various proteolytic fragments of CDB3 phosphorylated by casein kinase I and [δ-32P]ATP. CDB3 was phosphorylated by casein kinase I using radiolabeled ATP, as described in the Materials and Methods. Phosphorylated CDB3 was then either left unmodified or digested at specific sites and separated by Tricine SDS-PAGE. Lane A, intact CDB3; lane B, proteolytic fragments generated by NTCB cleavage; lane C, fragments produced by NH2OH hydrolysis; and lane D, fragments released upon digestion in formic acid. Lane E shows the position of molecular weight markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.3019/4/bl_0040f1.jpeg?Expires=1735830045&Signature=MLA0s50pPM-lyN-GFehcN3voWvULez-cV-XVqRKmEceKK2cflOZ1-c-GGyVzP7vC5habRqnYQnBXCOIH-67GqcbgRmBg50oGb5NNc80Ya8j-iPry9yOtJuHHF1ufD9dA6AHmGlSKmzfcJHTBFftwb5uQFIAClQNrr47DoAqs3MdienwZ6qRmmjbSZtrxD0sxgSjH0F7~i~R4uyFRpE-nnJXRNx5dMc8Q~Mj9k4iJi1G3OscyEiIHA6sb0nGAn6EERqT3oZw2dZZamYpACMbs-InaqNMxBdu53pCoDRJULiMRMZxQj0zsigW3ZcF7ouqPtdHNv29f1LUxdetrOvo2Aw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autoradiograph of various proteolytic fragments of CDB3 phosphorylated by casein kinase I and [δ-32P]ATP. CDB3 was phosphorylated by casein kinase I using radiolabeled ATP, as described in the Materials and Methods. Phosphorylated CDB3 was then either left unmodified or digested at specific sites and separated by Tricine SDS-PAGE. Lane A, intact CDB3; lane B, proteolytic fragments generated by NTCB cleavage; lane C, fragments produced by NH2OH hydrolysis; and lane D, fragments released upon digestion in formic acid. Lane E shows the position of molecular weight markers.
