Fig. 1.
Differential mRNA display of Mm subline cells. Total RNA isolated from Mm-A, Mm-P, Mm-S1, and Mm-S2 cells was reverse transcribed and amplified by PCR in the presence of [35S]dATP. AP-15 (5′-AGGGCCTGTT-3′ ) was used as an arbitrary primer and T12MT (A) or T12MC (B) was used as an anchored oligo(dT) primer. The PCR fragments were displayed on a 6% DNA sequencing gel and autoradiogrammed as detailed in the Materials and Methods. Fragments 15T01 (A) and 15C01 (B) are indicated by arrows.