Modulation of cell viability with a 48-hour treatment of M07e cells with p53-specific antisense oligonucleotides and Western blot analysis of the effects of p53 protein negation on the levels of protein products of p53-responsive genes in M07e cells. Following an 18-hour factor starvation period, 1 × 106 cells were treated for 48 hours with either Tpo (50 ng/mL), GM-CSF (GM; 100 U/mL), antisense oligonucleotide (5 μmol/L), control serum-free media, or sense oligonucleotide (5 μmol/L). (A) Cell viability was measured at 48 hours by trypan blue exclusion. Each data point was sampled in triplicate. (B) The levels of p53, Bax, Mdm-2, and Bcl-2 were analyzed by Western blot analysis as described in Materials and Methods. This figure represents data from one of three experiments which yielded similar results.
