Tpo induces an extranuclear buildup of p53 in M07e cells. After an 18-hour factor starvation period, 5 × 10⁶ cells were treated with either Tpo (50 ng/mL) or control serum-free media for 24 hours. Following this, the cell cultures were adjusted to contain 5 × 10⁷ cells/mL and divided into two groups. Nuclei were isolated from one of those groups and then adjusted to contain 5 × 10⁷ nuclei/mL. Thereafter, equal numbers of whole cells and nuclei from each treatment group were electrophoresed and analyzed by Western blot analysis. This figure represents data from one of three experiments which yielded similar results.