American Society of Hematology

Fig. 3.

$Fig. 3. Nuclear mobilization of Rel family proteins following stimulation of human neutrophils. Cells (5 × 106/mL) were cultured at 37°C in the presence of 1 μg/mL LPS (left panel) or 10 nmol/L fMLP (right panel); aliquots were taken at the indicated times (in minutes), and submitted to NP40 lysis. The resulting nuclear fractions were then analyzed by immunoblot using antisera to p50 (no. 1141), c-Rel, or p65/RelA (no. 1207); 106 cell-equivalents were loaded on the gels (except when probed for c-Rel, 7 × 105 cell-equivalents). The experiments depicted in this figure are representative of at least five.$

Nuclear mobilization of Rel family proteins following stimulation of human neutrophils. Cells (5 × 10⁶/mL) were cultured at 37°C in the presence of 1 μg/mL LPS (left panel) or 10 nmol/L fMLP (right panel); aliquots were taken at the indicated times (in minutes), and submitted to NP40 lysis. The resulting nuclear fractions were then analyzed by immunoblot using antisera to p50 (no. 1141), c-Rel, or p65/RelA (no. 1207); 10⁶ cell-equivalents were loaded on the gels (except when probed for c-Rel, 7 × 10⁵ cell-equivalents). The experiments depicted in this figure are representative of at least five.

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