Flow cytometry of the effect of dilazep on TF antigen expression on the surface of HUVECs stimulated with TNF, thrombin, or PMA. HUVECs were stimulated for 5 hours with each stimulant. (A) Control, (B) 1,000 U/mL TNF, (C) 25 nmol/L thrombin, and (D) 5 nmol/L PMA, in the presence (p) or absence (a) of 100 μg/mL dilazep. The cells were then harvested, incubated with murine monoclonal antihuman TF IgG, and then incubated with FITC-labeled goat antimouse IgG-antibody. Fluorescence intensity was determined by FACScan as described in the Materials and Methods, and the data are displayed using a logarithmic scale.