Effect of enzymatic treatment on P-selectin binding to CD24-coated beads. CD24-coated beads derived from PMNs were stained with human P-selectin–IgG and MoAb ML-5 against CD24 after treatment of the beads with endoglycosidase F to remove N-linked glycans or with OSGE from Pastorella hemolytica to cleave the protein at O-linked glycans. Samples treated with enzyme buffer alone did not show any change (not shown). Note that similar results were obtained with beads coated with CD24 antigen derived from SW2 or KS tumor cells.