Fig. 3.
Inhibitory effect of IL-10 on IL-2– or IL-15–mediated cytokine production. (A) T cells were isolated by rosetting and then stimulated by anti-CD3 + IL-15 (100 ng/mL) in the presence or absence of IL-10 (0.1 to 10 ng/mL). Depicted are mean and SEM of four independent experiments showing inhibition of cytokine-induced IFN-γ or TNF-α production. % Inhibition of cytokine production by IL-10 was calculated by (1 − [cytokine production in the presence of IL-10]/[cytokine production in the absence of IL-10]) × 100%. (B) T cells were isolated by rosetting and then stimulated by anti-CD3 + IL-2 (100 ng/mL) in the presence or absence of IL-10 (0.1 to 10 ng/mL). Depicted are mean and SEM of four independent experiments showing inhibition of cytokine-induced IFN-γ or TNF-α production. % Inhibition of cytokine production by IL-10 was calculated by (1 − [cytokine production in the presence of IL-10]/[cytokine production in the absence of IL-10]) × 100%.