Fig. 4.
![Fig. 4. Increasing concentrations of (A77 × 520C9) BsAb directed to FcαRI and HER-2/neu were analyzed in ADCC against SK-BR–3 breast cancer cells. As effector cell source, unfractionated whole blood (A, •) was compared with human plasma (▵), isolated MNC (○), or PMN effector cells (▪) (B) at an E:T ratio of 80 to 1. Significant ADCC (P < .05 indicated by an asterisk [*]) was observed with BsAb concentrations higher than 0.08 μg/mL in whole blood and with PMN, but not with plasma or MNC. Results from three experiments are presented as mean ± SEM of % specific lysis.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4485/3/bl_0031f4.jpeg?Expires=1735831536&Signature=c2T4NetO3AYxXuYP3cSBKr-3Fqflfr3BzS16fdHm-PFZUFy3nksqb135xxeJOQ9X2LeGCbkO1lE8yIJcCoxRmqt--UtfS3Ue9aa0MP~JiEpuzbMdnDdPBxUc1lWaJbd8LCaSAMhmwTFi26X7qBnP4qz4w3p51qU7R5sH04UZWSvU42hZSaSM0jSb-5BWMmfxtPv451FD1BGK7X12oLruQ2AF01AkHX5B3rNrIo0XsHr6c~POLnkZT6NxPLBSw3NYyioLFs0SauYhXG5S9Tr~ooMVEkVmZ5nZl36Kde~f2L4cV-HnSY3lqlVUAhD4qSkTSAl8Z591ad06ZtKaE05M1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Increasing concentrations of (A77 × 520C9) BsAb directed to FcαRI and HER-2/neu were analyzed in ADCC against SK-BR–3 breast cancer cells. As effector cell source, unfractionated whole blood (A, •) was compared with human plasma (▵), isolated MNC (○), or PMN effector cells (▪) (B) at an E:T ratio of 80 to 1. Significant ADCC (P < .05 indicated by an asterisk [*]) was observed with BsAb concentrations higher than 0.08 μg/mL in whole blood and with PMN, but not with plasma or MNC. Results from three experiments are presented as mean ± SEM of % specific lysis.
