Rocket immunoelectrophoresis of ferritin (A) and albumin (B) in hepatoma cell medium. (A) Rockets obtained with concentrated samples of conditioned medium from hepatoma cells in comparison with standards (Stds) of horse spleen ferritin (HSpFt) (3 slots on far right). Samples (6 lanes on the left) are from individual flasks of cells grown for 48 hours in protein-free medium (see Materials and Methods). Standards contained 10, 6, and 2 μg/mL horse spleen ferritin protein (from left to right). (B) Samples of medium from cells that were and were not treated with the Golgi function inhibitor, brefeldin A (+ BFA; 4 μg/mL), in comparison with rat albumin standards (Stds; 3 lanes on far right). Lanes 1 to 3 and 7 to 9 from left were from untreated cells, lanes 4 to 6 and 10 to 12 were from BFA treated cells, as indicated.