Effect of brefeldin A (1 μg/mL) on the release of ferritin from hepatoma cells, after stimulation with IL-1 or TNF (10 and 5 ng/mL, respectively). Bars show mean amounts of protein detected in the medium per mg cell protein, ± SD for 6 to 12 flasks per group, after 48 hours incubation, as a percent of mean values for the control (untreated) cells. Dark bars are without and lighter bars with brefeldin A. All differences with brefeldin treatment were significant.