Fig. 1.
WT1 gene (487 bp) and cABL gene (198 bp, control of RNA-integrity) RT-PCR amplification products of fetal blood MNCs and different single soft agar colonies are shown on an electrophoresis gel stained with ethidium bromide. Lanes from left: Fetal blood MNCs from patients S.M. (1), K.B. (2), and K.Z. (3), MNCs from the leukapheresis product of a solid cancer patient treated with stem cell-mobilizing chemotherapy and G-CSF4, colonies at day 14: CFU-GEMM (5), BFU-E (6), CFU-E (7), CFU-GEMM (8), colonies at day 21: BFU-E (9), CFU-GM (10), HL60 cells (11, positive control), water control (12), and marker lanes (ML).