RT-PCR SSCP analysis of the Fas antigen in myeloma patient BM specimens. ³²P-labeled PCR products are separated on a 6% acrylamide (49.5:0.5 bis) gel. Lane 1, PBLs; lane 2, 8226; lane 3, BAKA27 (normal); lane 4, FOSM32. Arrow indicates band excised for sequence analysis.