Fig. 1.
G-CSF increases the amount of B subunit in cytosol and membrane. Neutrophils were incubated for 3 hours in the presence or absence of G-CSF then disrupted by sonication. Membranes were obtained as the washed pellet of a 6-minute 100,000g centrifugation. The supernatant of the first centrifugation represented cytosol. Samples representing 2 × 107 cell equivalents were resolved by SDS-PAGE and detected with anti-B2 antibody4.
