RT-PCR analysis of lineage-sorted (A) and cytokine-treated (B) murine BM cells. (A) Bone marrow cells from C57BL/6 mice were sorted for RT-PCR to detect FAK mRNA (upper panel) and β-actin mRNA (lower panel). The MoAbs used in sorting were anti-c-Kit (lane 1), Gr-1 (lane 2), Mac-1 (lane 3), TER-119 (lane 4), CD4 (lane 5), CD8 (lane 6), and B220 (lane 7). (B) Day 7 GM-treated cells (lane 3, adherent cells; lane 4, nonadherent cells) and IL-3–treated cells (lane 5, adherent cells; lane 6, nonadherent cells) were subjected to RT-PCR to detect FAK mRNA (upper panel) and β-actin mRNA (lower panel). Unstimulated controls were also analyzed (lane 1, adherent cells; lane 2, nonadherent cells).