(A) Differential counts of GM-treated cells and IL-3–treated cells. Adherent and nonadherent cells were recovered on day 7 and the cytospin slides were subjected to May-Grünwald-Giemsa staining. (B) FCM analysis of murine BM cells after GM-CSF or IL-3 stimulation. The nonadherent BM-derived cells were analyzed for F4/80 expression (left panels) and c-Fms expression (right panels). Cells were harvested on day 0 (top panels), and on day 7 of either GM-CSF (middle panels) or IL-3 (bottom panels) stimulation. Solid line, stained with F4/80-FITC or anti-c-Fms-FITC; dotted line, unstained.