HGF-SF and c-met expression in primary cell cultures derived from peripheral blood cells. Northern hybridization analysis of total RNA (20 μg) from PBMNCs isolated from peripheral blood of healthy individuals for HGF-SF (A) and c-met (B) (lanes: respective positive controls (K562 or JVM-2), primary isolated PBMNCs from two healthy individuals (PBMNC [1] and [2]), primary culture of PBMNCs after 5 days (PBMNC [5D]), PBMNCs stimulated with PHA (PHA), PWM (PWM), or PHA, PWM, and LPS (PHA + PWM + LPS). Rehybridization for GAPDH is shown below. (C) Kinetic analysis of c-met expression under different conditions of stimulation. (), 1 and 2 hours; (▪), 6 hours; (□), 20 hours. Data represent evaluation of two independent Northern hybridizations with highest expression arbitrarily set to 1.