Phenotyping of cells generated in response to FL + IL-7 or FL + IL-7 + IL-10. A total of 500 Lin⁻Sca-1⁺c-kit⁺ cells was seeded in complete IMDM in the presence of optimal concentrations of FL + IL-7 (left panels) or FL + IL-7 + IL-10 (right panels). After 12 days of incubation, cells were harvested and examined for expression of B220, CD43, CD24, and GR-1, as described in the Materials and Methods. Staining with the antigen-specific antibodies (black) was compared with that of irrelevant isotype-matched control antibodies (gray). Data are from one (of four) representative experiments. For all panels, the x-axis represents relative logarithmic fluorescence intensity and the y-axis represents relative cell number.