Fig. 5.
![Fig. 5. IL-10 inhibits FL + IL-7–stimulated proB cell proliferation. A total of 500 Lin−Sca-1+c-kit+ cells was seeded in complete IMDM in the presence of FL + IL-7. After 12 days incubation, 1 × 105 of the generated B220+ cells were replated in triplicates in complete IMDM in medium alone, FL + IL-7, or FL + IL-7 + IL-10, as indicated. Cells were incubated for 48 hours and pulsed with 1 μCi [3H]-TdR during the last 12 hours of incubation. Samples were harvested and counted in a beta-counter as described in the Materials and Methods. Results represent the mean cpm (SEM) from three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4321/3/bl_0029f5.jpeg?Expires=1735501422&Signature=diuUBKu4OaQFdbdIeLG455QY6D5TUtIJ4J8Q4TCu~sU6yYvHyeiFMna3lkoRkM8dFp4B-0xbd6PzXQPmtkeCNp~IdiyT6hx96juEHmk9GNgWcsU98c8NNhmTU2Z3t26GYj8xL5QWOjnTbzArwnKFoegV8MhtvCTo6xJM09GQvAcKd9lbIMEmY~WB8fz3xRWk055BtBGRlOueVK4IT~YpAGG0PxYS-QxWbWESlwJ08lVnybWO~hxuIJDEmQYr6weSvvMfnYYFkS9T7dIIjPEdjHNlg3PCekPp5gbWemCyv49oPhZxwX4R9Mv0h1dYdBfSwXTQd9ykQl7C8DTMU~WZ2g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-10 inhibits FL + IL-7–stimulated proB cell proliferation. A total of 500 Lin−Sca-1+c-kit+ cells was seeded in complete IMDM in the presence of FL + IL-7. After 12 days incubation, 1 × 105 of the generated B220+ cells were replated in triplicates in complete IMDM in medium alone, FL + IL-7, or FL + IL-7 + IL-10, as indicated. Cells were incubated for 48 hours and pulsed with 1 μCi [3H]-TdR during the last 12 hours of incubation. Samples were harvested and counted in a beta-counter as described in the Materials and Methods. Results represent the mean cpm (SEM) from three independent experiments.
