Immunofluorescence analysis on murine Lin⁻c-kit⁺ HPCs cultured for 14 days in the presence of mouse TNFα, GM-CSF, and SCF. Three-color immunofluorescence analyses were performed as described in the Materials and Methods on the cultured HPCs at day 14. The indicated FITC-labeled MoAbs (CD8, Thy-1, CD11a, CD11b, and CD11c) were used to demonstrate the phenotype characteristics of the generated dendritic cells by gating on Ia⁺DEC-205⁺ or Ia⁺CD86⁺ cell population. Solid and dotted lines indicate the immunofluorescence intensity of cells stained with a control and the test antibodies, respectively. Representative results from three independent experiments are shown.