Plg deficiency reduces primary tumor size, skin ulceration, and hemorrhaging of Lewis lung carcinoma. (A) Representative examples of the macroscopic appearance of primary tumors in a Plg^−/− mouse (left panels) and a control mouse (right panels) at day 6 (upper panel) and day 8 (lower panel) after tumor cell injection. Note that the tumor in the Plg^−/− mouse is markedly smaller and displays reduced skin redness. (B) Representative examples of subcutaneous hemorrhaging in a Plg^−/− mouse (left) and a control mouse (right). Mice were killed at day 11 and perfusion fixed with paraformaldehyde as described in Materials and Methods, and the primary tumors were excised and photographed with the base of the tumor facing up. Note the marked reduction in free blood in the Plg^−/− mouse.