DTSSP cross-linking of TF on HL-60 cells. details of the DTSSP cross-linking and the Western blot analysis are presented in Material and Methods. Each lane contained the TF immunopurified from 5 × 10⁷ cells. The immunopurified TF samples were run unreduced on a 10% acrylamide Laemmli gel in the following order: lane 1, untreated cells (no DTSSP); lane 2, untreated cells + DTSSP; lane 3, ionomycin-treated cells + DTSSP; ionomycin-treated cells (pretreated with CMZ) + DTSSP.