Fig. 3.
Fig. 3. Immunostaining of human tissues and tonsil cell preparations using anti-HRX antibodies. (a) Antibody HRX 107 labels all nuclei in the thyroid gland in a punctate nuclear pattern. The pattern of labeling can be seen more clearly at high power (b). (c) MoAb HRX 107 labels most nuclei in the cerebellum in a punctate nuclear pattern, although the intensity of labeling varies from cell to cell. Again the pattern of labeling can be seen more clearly at high power, particularly the nuclear labeling of Purkinje cells (d). (e and f ) The punctate distribution of HRX in nuclei of cardiac myocytes found with MoAb HRX 107 which is comparable to the pattern of labeling with polyclonal antibody αHRX2 (g). (h) Labeling of nuclei in the glomerulus of kidney with MoAb HRX 107; the punctate pattern of distribution being clearly visible at high power (i). (j) Labeling of liver with polyclonal antibody αHRX2 and at high power (k), compared with MoAb HRX 107 (l). (m and n) Labeling of lung with polyclonal antibody αHRX2. (o) MoAb HRX 107 (red) labeling T cells (green). (p) MoAb HRX 107 (red) labeling B cells (green). (q) MoAb HRX 107 (red) labeling a macrophage cell (green). / Fig 4. Comparison of HRX with other known transcription factors that exhibit punctate nuclear staining. (a) MoAb HRX107 (red) and anti-TAL-1 (green) on the Jurkat cell line. (b) MoAb HRX107 (red) and anti-PML (green) on tonsil cell cytospins. (c) MoAb HRX107 (red) and anti-PML (green) on the NB4 cell line. / Fig 5. Comparison of MoAb HRX 107 and polyclonal antibody αHRX2 on cell lines. MoAb on U937 (a) and RS4; 11 (c). Polyclonal antibody on U937 (b) and RS4; 11 (d). / Fig 6. (a) Immunofluorescent labeling of Bosc 293 cells transfected with a flag-tagged HRX-ENL expression construct under control of the early CMV promoter and enhancer using MoAb HRX 107. (b) Immunofluorescent labeling of the same cells illustrated in 4a with the antiflag antibody. (c) Colocalization of MoAb HRX 107 and the antiflag antibody on the same cells illustrated in 4a and 4b. The untransfected cells (blue) can clearly be seen.

Immunostaining of human tissues and tonsil cell preparations using anti-HRX antibodies. (a) Antibody HRX 107 labels all nuclei in the thyroid gland in a punctate nuclear pattern. The pattern of labeling can be seen more clearly at high power (b). (c) MoAb HRX 107 labels most nuclei in the cerebellum in a punctate nuclear pattern, although the intensity of labeling varies from cell to cell. Again the pattern of labeling can be seen more clearly at high power, particularly the nuclear labeling of Purkinje cells (d). (e and f ) The punctate distribution of HRX in nuclei of cardiac myocytes found with MoAb HRX 107 which is comparable to the pattern of labeling with polyclonal antibody αHRX2 (g). (h) Labeling of nuclei in the glomerulus of kidney with MoAb HRX 107; the punctate pattern of distribution being clearly visible at high power (i). (j) Labeling of liver with polyclonal antibody αHRX2 and at high power (k), compared with MoAb HRX 107 (l). (m and n) Labeling of lung with polyclonal antibody αHRX2. (o) MoAb HRX 107 (red) labeling T cells (green). (p) MoAb HRX 107 (red) labeling B cells (green). (q) MoAb HRX 107 (red) labeling a macrophage cell (green).

Fig 4. Comparison of HRX with other known transcription factors that exhibit punctate nuclear staining. (a) MoAb HRX107 (red) and anti-TAL-1 (green) on the Jurkat cell line. (b) MoAb HRX107 (red) and anti-PML (green) on tonsil cell cytospins. (c) MoAb HRX107 (red) and anti-PML (green) on the NB4 cell line.

Fig 5. Comparison of MoAb HRX 107 and polyclonal antibody αHRX2 on cell lines. MoAb on U937 (a) and RS4; 11 (c). Polyclonal antibody on U937 (b) and RS4; 11 (d).

Fig 6. (a) Immunofluorescent labeling of Bosc 293 cells transfected with a flag-tagged HRX-ENL expression construct under control of the early CMV promoter and enhancer using MoAb HRX 107. (b) Immunofluorescent labeling of the same cells illustrated in 4a with the antiflag antibody. (c) Colocalization of MoAb HRX 107 and the antiflag antibody on the same cells illustrated in 4a and 4b. The untransfected cells (blue) can clearly be seen.

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