Time course of CP-10, MRP-14, and MCP-1 mRNA induction by LPS or IL-1β. bEnd-3 cells were cultured with LPS (1 μg/mL) or IL-1β (340 U/mL) for the indicated times and total RNA was analyzed by Northern blotting using CP-10, MRP-14, and MCP-1 riboprobes and the 18S rat RNA probe. Separate experiments with IL-1 and LPS are given. RNA was quantitated by densitometry and values expressed as detailed in the Materials and Methods. Results are representative of two experiments.