The state of confluence of bEnd-3 cells influences CP-10 mRNA expression. (A) mRNA from confluent, postconfluent, and late postconfluent bEnd-3 cells (days 0, 2, and 4, respectively) stimulated with LPS (1 μg/mL) for 24 hours. Quantitation by densitometry of CP-10 mRNA is detailed in the Materials and Methods. (B) bEnd-3 cells were subcultured onto 100-mm tissue culture plates at the densities given and grown for 4 days before stimulating with LPS (1 μg/mL) for 24 hours and mRNA was processed for Northern analysis. Results are representative of two experiments.