(A) Northern analysis showing CP-10 mRNA expression in bEnd-3 cells activated for 24 hours with LPS (1 μg/mL) or IL-1β (3.4 U/mL) with and without TNFα (12 U/mL). Confluent (day 0), postconfluent (day 2), and late postconfluent (day 4) monolayers were used. Blots were rehybridized with 18S probe as shown. Results are representative of two experiments. (B) CP-10 mRNA expression in bEnd-3 cells after LPS priming (0.01 to 10 ng/mL) for 16 hours, followed by stimulation with LPS at 100 ng/mL for 24 hours. CP-10 mRNA levels determined by densitometry are expressed relative to the nonprimed LPS-stimulated mRNA level (100%).