Expression of CP-10 mRNA is dependent on de novo protein synthesis. Northern blot analysis of CP-10 mRNA levels in bEnd-3 cells incubated for 24 hours without stimulation (control), with CHX (0.5 μg/mL), with LPS alone (100 ng/mL), with LPS and CHX, with IL-1β alone (30 U/mL), and with IL-1β and CHX. Blots were reprobed with the 18S RNA probe. Note that the results of different experiments with LPS and IL-1 are given. Results are representative of two experiments.