sIL-11R–stimulated tyrosine phosphorylation of gp130, STAT 3, and SHP-2. Western blot of cell lysates following immunoprecipitation with either (A) anti-LIF receptor antibody or (B through D) antiphosphotyrosine antibody. Blots were then probed with (A) antiphosphotyrosine, (B) anti-gp130, (C) anti-STAT 3, or (D) anti-SHP-2 antibodies. Lysates are from parental M1 cells in the presence of saline, LIF (1,000 U/mL), IL-6 (100 ng/mL), oncostatin M (100 ng/mL), IL-11 (5 nmol/L), sIL-11R plus IL-11 or sIL-11R alone (40 nmol/L). Samples were run on 8% to 25% SDS-PAGE.