American Society of Hematology

Fig. 7.

$Fig. 7. IL-11 antagonism by monomeric and aggregated forms of sIL-11R. (A) Western blot of sIL-11R fractions following gel filtration by Superdex 75 column (Pharmacia). For the bioassay, fractions 16 and 17 were used for the high moelcular weight form and fractions 20 to 22 for the monomeric form. Molecular weight markers are shown on the left side. (B) 3H-thymidine incorporation (1 μCi/well) measuring the response of M1/IL-11R cells to sIL-11R in the absence (□) or presence (▪) of 10 pmol/L IL-11. The monomeric form of sIL-11R is the solid line and the high molecular weight form is the broken line.$

IL-11 antagonism by monomeric and aggregated forms of sIL-11R. (A) Western blot of sIL-11R fractions following gel filtration by Superdex 75 column (Pharmacia). For the bioassay, fractions 16 and 17 were used for the high moelcular weight form and fractions 20 to 22 for the monomeric form. Molecular weight markers are shown on the left side. (B) ³H-thymidine incorporation (1 μCi/well) measuring the response of M1/IL-11R cells to sIL-11R in the absence (□) or presence (▪) of 10 pmol/L IL-11. The monomeric form of sIL-11R is the solid line and the high molecular weight form is the broken line.

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