American Society of Hematology

Fig. 4.

$Fig. 4. Patterns of human γ and β globin gene expression in β globin YAC transgenic lines during ontogeny. Human β-like globin RNA transcripts were quantified by primer extension and PhosphorImager analysis, and the results of multiple experiments were averaged. The level of activity of human γ or β globin genes is expressed as the fraction of total human ɛ, γ, and β globin gene expression at each gestational stage, corrected for the specific activity of the primers. Solid gray line, A20.1; broken gray line, A85.68; solid black line, A85.73; broken black line, A85.62. In line A20.1, the yolk sac was harvested on day 11.5 for these experiments. The pattern of γ globin gene expression is denoted with squares, and human β globin gene expression is designated by circles.$

Patterns of human γ and β globin gene expression in β globin YAC transgenic lines during ontogeny. Human β-like globin RNA transcripts were quantified by primer extension and PhosphorImager analysis, and the results of multiple experiments were averaged. The level of activity of human γ or β globin genes is expressed as the fraction of total human ɛ, γ, and β globin gene expression at each gestational stage, corrected for the specific activity of the primers. Solid gray line, A20.1; broken gray line, A85.68; solid black line, A85.73; broken black line, A85.62. In line A20.1, the yolk sac was harvested on day 11.5 for these experiments. The pattern of γ globin gene expression is denoted with squares, and human β globin gene expression is designated by circles.

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