Nucleotide sequence of MARCO cDNA. The entire nucleotide sequence of the isolated cDNA clone, MARCO, is shown in (a). The nucleotide sequences of murine mitochondrial DNA fragment and rat mitochondrial cytochrome c oxidase/serine tRNA cDNA are shown in (b) and (c), respectively (from 3′ to 5′ direction) with the corresponding complementary sequence of MARCO. The nucleotide of MARCO is numbered from 5′ terminal. In each sequence profile, mismatched nucleotides between MARCO (a) and complementary nucleotide sequences of murine mitochondrial DNA fragment (b) are demonstrated with bold typing. The 3′ end of the serine tRNA region is indicated with an arrow. The PvuII site used to prepare the probe for northern blotting analysis is indicated with an arrowhead. The horizontal arrows indicate primers used for RT-PCR analysis (rightward direction for sense primer and leftward direction for antisense primer).