RT-PCR analysis of MARCO transcript. (A) Lane 1, MW marker; 2, 3, 4, 5, 14, 15, 16, and 17, wild-type HL-60 cells; 6, 7, 8, and 9, mock-transfected HL-60 cells; 10, 11, 12, and 13, NF-1 cells; 2, 4, 6, 8, 10, 12, 14, and 16, without, and 3, 5, 7, 9, 11, 13, 15, and 17, with reverse transcriptase; 2, 3, 6, 7, 10, and 11, without, and 4, 5, 8, 9, 12, and 13, with IPTG for 6 hours; 14 and 15, with RA for 48 hours; 16 and 17, with RA for 84 hours. (B) Lane 1, MW marker; 2, NF-1 cells; 3, 4, 5, and 6, NFS-60 cells; 7, 8, 9, and 10, TF-1 cells; 11, 12, 13, and 14, K562 cells; 3, 5, 7, 9, 11, and 13, without, and 2, 4, 6, 8, 10, 12, and 14, with reverse transcriptase; 3 and 4, with, and 5 and 6, without rhG-CSF for 6 hours; 7 and 8, with, and 9 and 10, without rhGM-CSF for 6 hours; 11 and 12, without, and 13 and 14, with hemin for 96 hours. (C) Lane 1, MW marker; 2 and 3, human bone marrow nonadherent mononuclear cells; 4 and 5, human blood lymphocytes; 6 and 7, human blood neutrophilic granulocytes; 8, NF-1 cells; 2, 4, and 6, without, and 3, 5, 7, and 8, with reverse transcriptase; 8, with IPTG for 6 hours. (D) Lane 1, MW marker; 2, 3, 4, 5, 6, 7, 8, and 9, human blood neutrophilic granulocytes; 2, 4, 6, and 8, without, and 3, 5, 7, and 9, with reverse transcriptase; 2, 3, 6, and 7, incubated for 3 hours, and 4, 5, 8, and 9, incubated for 6 hours; 2, 3, 4, and 5, without, and 6, 7, 8, and 9, with rhG-CSF.