Bryo-1 enhances IL-2–induced expression of IL-1β, TNF-α, IL-6, and IL-8 mRNA. Monocytes were incubated for 3 hours with IL-2 (1,000 U/mL) alone or in combination with 0.1 or 0.01 ng/mL of bryo-1 before total RNA was isolated and sequentially probed for cytokine mRNA levels by Northern blot. The blot was then rehybridized with the GAPDH probe to check the RNA loading. These data are representative of three different experiments.